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Image reconstruction of low and high dose micrographs of negatively stained
HIGH VOLTAGE ELECTRON MICROSCOPY
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acid alveolar antibody arrows axons biological buffer calcium capillary Cell Biol cell membrane cell surface cell wall cellular chromatin coated collagen complex containing crystals culture cytochemical cytoplasm demonstrated dense density Department of Anatomy diameter diffraction electron micrographs electron microscopy embedded endoplasmic reticulum endothelial enzyme Epon ferritin fibrils Fig.l Figure filaments fixation fixed formation fracture freeze-etched freeze-fracture frozen glial glutaraldehyde Golgi Golgi apparatus granules grids hepatocytes incubated infected intracellular isolated labeled layer lead citrate lipid lysosomes magnification material matrix method microanalysis microtubules microvilli mitochondria molecules morphological muscle negatively stained normal nuclear nucleolus nucleus observed organelles osmium osmium tetroxide particles plasma membrane plasmalemma present processes protein reaction replicas ribosomes scanning electron secretory seen specimen structure subunits technique temperature thick thin sections tight junctions tion tissue transmission electron transmission electron microscopy tubules ultrastructural ultrathin uranyl acetate vacuoles vesicles virus X-ray