Experiments in Plant Tissue Culture

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Cambridge University Press, Jan 27, 1995 - Science - 256 pages
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In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations have been added, together with new experiments on such topics as potato callus formation, and embryo culture. This book will be useful to graduate students and professionals in plant science, forestry, agronomy, and horticulture.
 

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Contents

Laboratory facilities
19
Aseptic techniques
25
Media composition and preparation
42
Initiation and maintenance of callus
69
Organogenesis
82
Cell suspensions
92
Somatic embryogenesis
101
Culture of organs and organized systems
115
Anther and pollen cultures
136
Transdifferentiation of parenchyma cells to tracheary
155
Isolation and culture of protoplasts
167
Protoplast fusion and somatic hybridization
183
Production of secondary metabolites
204
Abbreviations
229
Author index
243
Copyright

Micropropagation by bud proliferation
126

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