Experiments in Plant Tissue Culture
The third edition of Experiments in Plant Tissue Culture makes available new and updated information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This laboratory text features an enlarged section on terminology and definitions as well as additional information on equipment and facilities. This comprehensive text takes the reader through a graded series of experimental protocols and also provides a preliminary review of each topic. After an introductory historical background, there are discussions of a plant tissue culture laboratory, aseptic techniques, and nutritional components of media. Subsequent chapters are devoted to callus induction, organ formation, cell suspensions, somatic embryogenesis, root cultures, micropropagation, anther and pollen cultures, xylem cell differentiation, isolation and fusion of protoplasts, storage of genetic resources, secondary metabolite production, and quantitation of procedures. A glossary of terms, a list of commercial sources of supplies, and a table of media formulations are included. This book offers all the basic experimental methods for the major research areas of plant tissue culture, and will be invaluable to undergraduates and research investigators in the plant sciences. It will also be useful to researchers in related areas such as forestry, agronomy, and horticulture, and to those in commercial houses and pharmaceutical companies interested in acquiring the laboratory techniques.
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Media composition and preparation
Initiation and maintenance of callus
Culture of organs and organized systems
Anther and pollen cultures
Transdifferentiation of parenchyma cells to tracheary
Isolation and culture of protoplasts
Protoplast fusion and somatic hybridization
Production of secondary metabolites
Micropropagation by bud proliferation
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acid addition agar anther culture anthers apices aseptic autoclave auxin buds callus carrot cell cultures cell division cell wall Chapter chemical Cocking coconut concentration containing culture medium culture tubes cytokinin DDH2O differentiation embryogenesis embryogenic embryoids embryos enzyme Erlenmeyer flasks ethanol ethanol 70 excised experiment explants filter paper forceps formation Fukuda fusion genetic growth regulators haploid haploid plants hybrid hypochlorite IAPTC induced initiation isolated protoplasts kinetin Komamine laboratory leaf liquid medium mannitol mesophyll mesophyll cells micropropagation microscope mixture morphogenesis Murashige nutrient organ organogenesis oven P.O. Box Pasteur pipette Petri dish pipette plant cell plant growth plant material Plant Physiol plant tissue culture plantlets pollen grains potato preparation procedure production propagation protoplasts regeneration Reinert remove rinse roots sample scalpel secondary metabolites single cells Skoog somatic embryogenesis species subculture sucrose surface sterilization techniques temperature tion tobacco tracheary elements transdifferentiation transferred ture vitamin vitro xylogenesis