Experiments in Plant Tissue Culture
In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations have been added, together with new experiments on such topics as potato callus formation, and embryo culture. This book will be useful to graduate students and professionals in plant science, forestry, agronomy, and horticulture.
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Media composition and preparation
Initiation and maintenance of callus
Culture of organs and organized systems
Anther and pollen cultures
Transdifferentiation of parenchyma cells to tracheary
Isolation and culture of protoplasts
Protoplast fusion and somatic hybridization
Production of secondary metabolites
Micropropagation by bud proliferation
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acid addition agar anther culture anthers apices aseptic autoclave auxin buds callus carrot cell cultures cell division cell wall Chapter chemical Cocking coconut concentration containing culture medium culture tubes cytokinin DDH2O differentiation embryogenesis embryogenic embryoids embryos enzyme Erlenmeyer flasks ethanol ethanol 70 excised experiment explants filter paper forceps formation Fukuda fusion genetic growth regulators haploid haploid plants hybrid hypochlorite IAPTC induced initiation isolated protoplasts kinetin Komamine laboratory leaf liquid medium mannitol mesophyll mesophyll cells micropropagation microscope mixture morphogenesis Murashige nutrient organ organogenesis oven P.O. Box Pasteur pipette Petri dish pipette plant cell plant growth plant material Plant Physiol plant tissue culture plantlets pollen grains potato preparation procedure production propagation protoplasts regeneration Reinert remove rinse roots sample scalpel secondary metabolites single cells Skoog somatic embryogenesis species subculture sucrose surface sterilization techniques temperature tion tobacco tracheary elements transdifferentiation transferred ture vitamin vitro xylogenesis