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A RNA Extraction
RTPCR Without RNA Isolation
43 other sections not shown
1999 BioTechniques Books A.B. Pardee agarose gel anchor primer annealing temperature arbitrary primer Arthur Pardee assay BioTechniques Cancer cDNA fragments cells cloning concentration containing cycles DDRT-PCR products denaturing described detected differential display Differential Display Edited differentially expressed genes DMSO DNA sequencing DNase dNTP electrophoresis enzyme Expression Genetics false positives Figure fingerprinting fluorescent gene expression genomic DNA hybridization identified incubated isolated labeled lane Liang McClelland 1999 BioTechniques membrane merase messenger RNA method MgCl2 Michael McClelland 1999 mixture Molecular mRNA differential display multiplex PCR Northern blot Nucleic Acids Nucleic Acids Res nucleotides oligo(dT oligonucleotides optimal Pardee and Michael PCR amplification PCR buffer PCR products performed plasmid polymerase chain reaction primer pairs probes procedure Promega protein protocol purified quantitative reamplification reverse transcriptase RNase RNase inhibitor RT-PCR selected sequencing gel specific step Taq DNA polymerase target template tion tissue total RNA transcripts Tris-HCl tube tumor vector