FRET and FLIM Techniques
Theodorus W. J. Gadella
Elsevier, Jul 29, 2011 - Science - 560 pages
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
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Frequency domain FLIM theory instrumentation and data analysis
Time domain FLIM Theory instrumentation and data analysis
Multidimensional fluorescence imaging
Visible fluorescent proteins for FRET
Small moleculebased FRET probes
FilterFRET Quantitative imaging of sensitized emission
Spectral imaging and its use in the measurement of Foumlrster resonance energy transfer in living cells
acceptor photobleaching acquisition analysis applications autofluorescence avGFP Biochem Biol biological Biophys calculated capillary cence Cerulean channel chapter Chem chromophore confocal confocal microscopes coumarin detection detector dipole diVerent donor and acceptor donor fluorescence dyes dynamics electron emission spectra enzyme eVects excitation wavelength filter FLIM fluores fluorescein fluorescence decay fluorescence intensity fluorescence lifetime imaging fluorescence microscopy fluorescence resonance energy fluorophores Fo¨rster frequency domain FRET eYciency FRET imaging FRET pair FRET probes FRET‐based Gadella green fluorescent protein interactions Jovin labeling laser lifetime imaging microscopy linear unmixing lipid living cells measure FRET membrane methods Microsc modulation molecular molecules mutations optical panel pathway peptide photobleaching photon pixel protoplasts pulse quantitative quantum yield quenching ratio resonance energy transfer sample sensitized emission sensors signal spatial spectral images spectroscopy spectrum TCSPC techniques time‐gated tion TIRF Tsien two‐photon variants Venus VFPs wide‐field ţ ţ