FRET and FLIM Techniques

Front Cover
Theodorus W. J. Gadella
Elsevier, Jul 29, 2011 - Science - 560 pages
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This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
 

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Contents

Foumlrster resonance energy transferFRET what is it why do it and how its done
1
Frequency domain FLIM theory instrumentation and data analysis
59
Time domain FLIM Theory instrumentation and data analysis
95
Multidimensional fluorescence imaging
133
Visible fluorescent proteins for FRET
171
Small moleculebased FRET probes
225
FilterFRET Quantitative imaging of sensitized emission
289
Spectral imaging and its use in the measurement of Foumlrster resonance energy transfer in living cells
351
Total internal reflection fluorescence lifetime imaging microscopy
395
FRET and FLIM applications in plants
413
Biomedical FRETFLIM applications
447
Reflections on FRET imaging Formalism probes and implementation
475
Glossary
519
Index
521
Color Plate Section
535
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