Fertility Cryopreservation

Front Cover
Ri-Cheng Chian, Patrick Quinn
Cambridge University Press, May 13, 2010 - Medical - 288 pages
Protecting the reproductive potential of young patients undergoing cancer therapy is increasingly important. With modern treatment protocols, 80% of patients can be expected to survive. It has been estimated that up to one in 250 young adults will be a survivor of childhood cancer in the future; infertility, however, may be a consequence. As a wide range of fertility preservation methods are increasingly offered by clinicians, this systematic and comprehensive textbook dealing with the cryobiology, technology and clinical approach to this therapy will be essential reading to infertility specialists, embryologists, oncologists, cryobiologists, ObGyns, andrologists, and urologists with an interest in fertility preservation. Fertility Cryopreservation reviews all the techniques of this increasingly important field within reproductive medicine. It covers the basic principles of pertinent cryobiology, and contains major sections on the different therapies available, written by international specialists combining experience from both academic centers and commercial industries.
 

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Contents

1 Cryobiology
1
2 Suppression of ice in aqueous solutions
10
3 Movement of water and cryoprotectants in mouse oocytes and embryos at different stages relevance to cryopreservation
16
4 Cryoprotectants
24
5 Cryopreservation of sperm and testicular tissue
39
6 Sperm cryopreservation for a donor program
46
7 Cryopreservation of surgically retrieved sperm
51
8 Testicular tissue cryopreservation
57
16 Vitrification of human oocytes with different tools
131
17 Vitrification of human oocytes using the McGill Cryoleaf protocol
144
18 Cryopreservation of human oocytes and embryos either by direct plunging into liquid nitrogen or by using an aseptic approach
157
19 Vitrification of human oocytes for a donor program
169
20 Obstetric and perinatal outcomes in pregnancies conceived following oocyte cryopreservation
178
21 Cryopreservation of ovarian tissue
189
22 In vitro culture of human primordial follicles
200
23 Concept of human ovarian tissue
213

9 Cryopreservation of embryos
67
10 Cryopreservation of pronuclear stage human embryos
76
11 Cryopreservation of day two and day three embryos
89
12 Cryopreservation of blastocysts
95
13 Aseptic vitrification of human blastocysts
106
14 Cryopreservation of oocytes
114
15 Cryopreservation of oocytes by slow cooling
120
24 Transplantation of cryopreserved ovarian tissues
218
25 Whole ovary cryopreservation
233
26 Transplantation of whole frozenthawed ovaries
241
27 Ethical considerations
248
Appendix Autotransplantation of cryopreserved thawed human ovarian tissue
257
Index
260
Copyright

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About the author (2010)

Ri-Cheng Chian is Scientific Director at the McGill Reproductive Center, McGill University Health Center and Assistant Professor in the Department of Obstetrics and Gynecology at McGill University, Montreal, Canada.
Patrick Quinn is Vice-President, Research and Development, Sage IVF, CooperSurgical Company, Trumbull, Connecticut, USA.

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