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Factors Affecting the Quality of Fixation
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0.1 mol/1 cacodylate acrolein aldehyde amino acids amino groups animal aorta artifacts buffer pH 7.2 CaCl2 cacodylate cacodylate buffer pH cause cells cellular changes concentrations of glutaraldehyde containing cross-linking cytoplasm dehydration denaturation dialdehyde digitonin Distilled water DMSO duration of fixation effect enzyme enzyme activity extracellular extraction fixation with glutaraldehyde fixative solution formaldehyde formation glutaral glutaraldehyde glutaraldehyde-fixed glycogen granules hr at 4°C hr at room hypertonic immersion increase incubation indicated ions lipids method microtubules mitochondria mixture of glutaraldehyde mol/1 cacodylate buffer mol/1 sucrose molecules nucleic acids obtained organelles Osmium tetroxide osmolality osmolarity oxidation paraformaldehyde perfusion perfusion pressure phospholipids plasma membrane polymerization polymers postfixed presence preservation procedure proteins rate of penetration react reaction reactive reagent ribosomes room temperature shrinkage sodium soluble specimens are fixed staining structure studies sucrose surface swelling tannic acid tion tissue blocks tissue specimens ultrastructure uranyl acetate vascular perfusion vehicle washing whereas