Flow Cytometry Data Analysis: Basic Concepts and Statistics

Front Cover
Cambridge University Press, Sep 15, 2005 - Science - 300 pages
0 Reviews
Flow cytometry is now well established in research laboratories and is gaining increasing use in clinical medicine and pathology. The technique enables multiple simultaneous light scatter and fluorescence measurements to be made at the individual cell level at very rapid rates and results in very large quantities of data being collected. Data, however, is just a series of numbers which have to be converted to information which, in turn, must be shown to have meaning. This is the most important single aspect of flow cytometry but it has received relatively little attention. One of the frequently voiced advantages of the technology is that it produces 'good statistics' because large numbers of cells have been analysed. However, it is not very often that confidence limits are placed on results, hence the reader has little or no feel for the inherent variability in the information produced. This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analysing dynamic processes. All those who use flow cytometry in their research will find this book an invaluable guide to interpreting the data produced by flow cytometers.
 

What people are saying - Write a review

We haven't found any reviews in the usual places.

Contents

1 Introduction
1
2 Fundamental concepts
4
3 Probability functions
20
4 Significance testing and fit criteria
31
5 Regression analysis
58
6 Flow cytometric sources of variation
82
7 Immunofluorescence data
101
8 DNA histogram analysis
126
Numerical integrating routine
249
Normal distribution probabilities
250
Variance ratio tables
252
MannWhitney U tables
256
Appendix 5
262
Regression analysis for y on x
265
Appendix 7
266
Appendix 8
269

9 Cellcycle kinetics
145
10 Dynamic cellular events
197
11 Multivariate analysis primer
241
12 Epilogue
246
Appendix 9
272
References
275
Index
285
Copyright

Other editions - View all

Common terms and phrases

Bibliographic information