Flow Cytometry of Intracellular Calcium in Platelets
Document from the year 2012 in the subject Medicine - Pharmacology, , language: English, abstract: During the last years flow cytometry has been developed into an important diagnostic tool with a wide range of applications. Whereas the differentiation of leukocytes is most often encountered, flow cytometry also renders itself especially useful in the study of platelets. Commonly, surface markers are of key interest, but the fact that platelets can be conveniently activated in vitro offers the opportunity to investigate intracellular signal transduction processes, which lead to the release of calcium into the cytosol. And it is this rise of intracellular calcium that flow cytometry is able to detect in real time through the use of suitable calcium-sensitive markers. It is the aim of this book to put together an overview of this technique and therefore to provide a sound starting ground for its application to clinical and experimental investigations and also for its further development.
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The Use of Fluo3 as Intracellular Calcium Indicator
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absorption acetoxymethyl activated platelets adenosine adhesion aggregation allows Bernard-Soulier syndrome blood cells calcium channel coagulation collagen Colman RW cyclooxygenase cytometer cytosolic cytosolic free calcium dense bodies dense tubular system diacylglycerol dimethylsulfoxide Dotplot FSC dyes electrons emission spectra epinephrine ester fibrinogen fibrinogen receptor Figure FITC flow cytometry fluorescein isothiocyanate fluorescence fluorescence intensity fluorochromes forward scatter glycoprotein Gp IIb-IIIa receptors haemostatic plug Hemostasis Hemostasis and thrombosis Histogram plot inhibit interaction intracellular intracellular calcium concentration intracellular signal transduction isotype control leukocytes Lippincott Williams mainly measurement molecule monoclonal antibodies parameters peridinin chlorophyll protein phosphatidylinositol 4,5-bisphosphate phospholipase photomultiplier tube photons phycoerythrin PIP2 plasma membrane platelet activation Platelet Agonists principles and clinical release of calcium sample scatter SSC scattered laser light secretion shape change sheath fluid side scatter signal transduction signal transduction pathways soluble specific stimulation subendothelium substances thrombin thromboxane A2 TXA2 von Willebrand Factor wavelength Williams & Wilkins µmol/l