Flow Cytometry of Intracellular Calcium in Platelets
GRIN Verlag, 2012 - 68 pages
Document from the year 2012 in the subject Medicine - Pharmacology, language: English, abstract: During the last years flow cytometry has been developed into an important diagnostic tool with a wide range of applications. Whereas the differentiation of leukocytes is most often encountered, flow cytometry also renders itself especially useful in the study of platelets. Commonly, surface markers are of key interest, but the fact that platelets can be conveniently activated in vitro offers the opportunity to investigate intracellular signal transduction processes, which lead to the release of calcium into the cytosol. And it is this rise of intracellular calcium that flow cytometry is able to detect in real time through the use of suitable calcium-sensitive markers. It is the aim of this book to put together an overview of this technique and therefore to provide a sound starting ground for its application to clinical and experimental investigations and also for its further development.
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acetoxymethyl activated platelets adhesion analysis antigen blood cells Calcium in Platelets coagulation collagen Colman RW Cytometry of Intracellular cytosol cytosolic free calcium dense tubular system diacylglycerol dimethylsulfoxide Dotplot FSC dyes electrons emission spectra fibrinogen Figure FITC flow cytometry fluorescein isothiocyanate fluorescence fluorescence intensity fluorochromes forward scatter Gamsjäger Flow Cytometry glycoprotein Gp IIb-IIIa receptors haemostatic plug Hemostasis Hemostasis and thrombosis inhibit intracellular calcium concentration isotype control leukocytes Lippincott Williams markers measurement molecule monoclonal antibodies non-steroidal anti-inflammatory drugs parameters peridinin chlorophyll protein phosphatidylinositol 4,5-bisphosphate phospholipase photomultiplier tube photons phycoerythrin PIP2 plasma membrane platelet activation platelet function principles and clinical prostaglandin receptor activator peptide release of calcium sample secretion shape change sheath fluid signal transduction signal transduction pathways soluble specific subendothelium substances surface antigen Thomas Gamsjäger Flow Thromb thrombin receptor thrombin receptor activator thromboxane A2 TXA2 von Willebrand Factor wavelength whole blood Willebrand Factor Williams & Wilkins