Fly Pushing: The Theory and Practice of Drosophila GeneticsA second edition of the classic handbook has become a standard in the Drosophila field. This edition is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques. |
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LibraryThing Review
User Review - GrumpyBob - LibraryThingThis is perhaps *the* book to learn practical Drosophila (fruit fly) genetics. It is clearly written and leads the reader through chapters on the basics of crosses; how to isolate new mutants, genetic ... Read full review
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really good!
Contents
3 | |
4 | |
6 | |
CHAPTER 2 | 25 |
CHAPTER 3 | 53 |
Synthesizing Specific Genotypes | 77 |
MANIPULATING THREE CHROMOSOMES | 87 |
CHAPTER 5 | 101 |
INTERACTING LOCI | 109 |
MAINTENANCE OF MUTANT PHENOTYPES | 117 |
II Mosaics | 121 |
CHAPTER 7 | 147 |
Glossary | 159 |
References | 171 |
Index | 183 |
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Common terms and phrases
activity addition allele analysis approach autosomal balancer become carrying cells Chapter chromo chromosome clones cn bw collect combinations complementation construct containing cross culture deletions depends designated detectable determine dominant dominant marker dose Drosophila duplication effect element embryos et al event example excision expression fact females flies FM7a frequency function gene genetic genome genotype heterozygous homologs homozygous identified important indicates individual induced insertion interactions kind known lethal lines loci locus loss males marked marker mate means melanogaster mosaic mosome mutagenesis mutagenized mutation normal null obtained occur original P-element particular phenotype possible present problem produce progeny protein rearrangements recessive recombination recover refers region requires result scheme score screen segregation Select sequence simply single strains strategy technique third tion transgene usually vial virgins wild-type
Popular passages
Page 136 - in King Lear: The wren goes to't, and the small gilded fly Does lecher in my sight. Let copulation thrive...
Page 60 - on the recombinant chromosomes. If x is distal to the P element, the recombinant chromosomes m¿ m 2 do not complement x, and m 1
Page 60 - do complement x (shown here). If x is proximal to the P element, m,
Page 60 - identified by the presence of only the m 1 or m 2 visible marker,
Page 60 - and m 2 are proximal and distal flanking markers that flank the P element.
Page 60 - Flies with only the m 1 visible marker are homozygous for the mutation x.
Page 149 - Drosophila: A Laboratory Handbook, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring