Fly Pushing: The Theory and Practice of Drosophila GeneticsA second edition of the classic handbook has become a standard in the Drosophila field. This edition is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques. |
Contents
| 3 | |
| 4 | |
| 6 | |
CHAPTER 2 | 25 |
CHAPTER 3 | 53 |
DELETION MAPPING | 61 |
MAPPING PELEMENT INSERTION SITES | 69 |
Synthesizing Specific Genotypes | 77 |
INTERACTING LOCI | 109 |
MAINTENANCE OF MUTANT PHENOTYPES | 117 |
II Mosaics | 121 |
CHAPTER 7 | 147 |
Glossary | 159 |
References | 171 |
Index | 183 |
CHAPTER 5 | 101 |
Other editions - View all
Fly Pushing: The Theory and Practice of Drosophila Genetics Ralph J. Greenspan No preview available - 2004 |
Common terms and phrases
Acph analysis Ashburner attached-X autosomal balancer chromosome breakpoints cells centromere Chapter chro chromo classes of progeny clones cn bw complementation cross culture deletions dominant marker dose Drosophila Genome Drosophila melanogaster duplication embryos enzyme excision expression eye-color fly genetics FlyBase FM7a frequency gain-of-function GAL4 gametes gene gene product genome genotype Golic heterozygous homologous recombination homozygotes homozygous hypomorph In(2LR)O,Cy Sco induced interactions inversions lethal mutations loci locus m₁ m₂ male recombination marker mutation mate meiosis meiotic map mitotic recombination molecular mosaic mosome muta mutagenesis mutagenized mutant allele mutant phenotype mutant screen normal null allele obtained occur odd+ ơơ P-element P[w+ phenotype present problem produce protein recessive lethal recombination event region result ruPrica scheme score screen second chromosome segregation sequence sperm strains strategy target technique temperature-sensitive third chromosome tion transgene translocation transposable elements transposase transposon viable vial Wieschaus X chromosome
Popular passages
Page 136 - in King Lear: The wren goes to't, and the small gilded fly Does lecher in my sight. Let copulation thrive...
Page 60 - on the recombinant chromosomes. If x is distal to the P element, the recombinant chromosomes m¿ m 2 do not complement x, and m 1
Page 60 - do complement x (shown here). If x is proximal to the P element, m,
Page 60 - identified by the presence of only the m 1 or m 2 visible marker,
Page 60 - and m 2 are proximal and distal flanking markers that flank the P element.
Page 60 - Flies with only the m 1 visible marker are homozygous for the mutation x.
Page 149 - Drosophila: A Laboratory Handbook, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring