Foreign Gene Expression in Fission Yeast: Schizosaccharomyces Pombe
Yuko Giga-Hama, Hiromichi Kumagai
Springer, Oct 17, 1997 - Medical - 182 pages
Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.
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Expression and Secretion of Mammalian Proteins
Constitution of Expression Vectors
Exploiting the Thiamine Biosynthetic Gene mmti
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Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe
Yuko Giga-Hama,Hiromichi Kumagai
No preview available - 2014
Acad Sci USA activity amino acid antibody ars1 bacteriorhodopsin Biochem Biol Chem blot analysis budding yeast cDNA cell cycle cloning codon coli concentration containing copy number Curr Genet efficiency EMBO encoding enzymes eukaryotes Expression in Fission expression level expression systems expression vector FEBS Lett fission yeast Schizosaccharomyces Foreign Gene Expression functional G protein gene expression genome glucose glycosylation Golgi heterologous protein higher eukaryotes HindIII homologous human lipocortin kinase L1 protein lane LEU2 lipocortin mammalian cells Maundrell membrane proteins method microsomes Mol Cell Biol molecular mRNA mutants N-terminal Natl Acad Sci nmt1 nmti promoter P-factor pg/ml phosphatase plasmid pRL2L Proc Natl Acad protease protein coupled purified receptor recombinant proteins regulated replication repressed residues retinal rhodopsin Saccharomyces cerevisiae saccharomyces pombe Schizo SDS-PAGE SEAP secretion Secretion Vector selectable marker signal sequence sion structure TATA box teins thiamine tion transcription transformation ura4 Western blot yeast cells yeast Schizosaccharomyces pombe