Free Radicals: A Practical Approach
Neville A. Punchard, Frank J. Kelly
IRL Press at Oxford University Press, 1996 - Science - 310 pages
With the recognition that oxygen and nitrogen radicals are involved in normal cell metabolism, free radical research has begun to feature in most disciplines in the life sciences. Increasingly, their implication in a number of human disease processes is being accepted, altough conclusiveevidence is lacking in many instances, partly due to the difficulties in detecting and measuring free radicals that have lifetimes measured in microseconds. This book outlines the latest techniques for quantifying radicals and their effects, and features detailed protocols, hints, and tips forsuccess, troubleshooting comments, sample data, and key literature citations.
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In vitro and in vivo detection of free
Spin trapping detection of free radicals
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4HNE absorbance acetate aldehydes analysis anion antioxidant aqueous assay Biochem Biol biological fluids blood buffer centrifuge Chem chemical chemiluminescence chromatogram column compounds concentration cuvette derivatization detection detector determination dilution DNPH EDTA electron elution enzyme Equipment and reagents ex vivo extraction F2-isoprostanes Fe2+ filter fluorescence formation free radical Grootveld GSH peroxidase GSSG guanidine H NMR hexane homogenates HPLC HPLC-grade hydroperoxides injury ions isoprostanes kinetics L-NAME leucocytes lipid hydroperoxides lipid peroxidation liver measured membrane metabolism metabolites methanol method molecules nitrogen nmol NMR spectroscopy OFRs OH radical oxidative stress oxygen peak perfused peroxidase phenylalanine plasma probes protein Protocol pulse radiolysis quantification radical adducts reaction reactive reduced resonance room temperature salicylate samples sensitivity sodium solution solvent species spectra spectroscopy spin trapping superoxide synovial fluid technique tion tissue tocopherol tube vitamin Vladimirov wavelength