Fundamentals of Light Microscopy

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CUP Archive, Jul 1, 1982 - Science - 93 pages
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Dr Spencer's account gives, in largely non-mathematical terms, a concise but rigorous explanation of the principles underlying the various forms of light microscopy. It starts with the properties of lenses, elementary diffraction theory and theoretical limits to resolution; it goes on to explain the workings of phase contrast, dark-field, fluorescence, polarizing and interference microscopes. There are chapters on aberrations and photomicrography, and detailed instructions for the alignment and general care of microscopes. Questions and answers are given at the end of each chapter, and suggestions made for class experiments. Although written primarily for undergraduates, the treatment is equally suitable for those preparing for professional examinations in microscopy, and for research workers seeking a better understanding of the microscopes they use.
 

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Contents

Properties of light
9
The limits of resolution
18
The Abbe approach
24
Brightfield phase and darkfield microscopy
32
Fluorescence microscopy
40
Polarizing microscopy
46
Interference methods
54
Aberrations and microscope design
63
Photomicrography
70
Mathematical treatments
78
Index
87
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