Fundamentals of EnzymologyNow in a new edition, this book provides an up-to-date account of the field of enzymology. it describes the action of enzymes in systems of increasing complexity, and shows how these properties can be best utilized, both clinically and technically. The reader will find in-depth coverage purified enzymes, including full treatment of purification methods, structure, kinetics, mechanism of action, and the control of enzyme activity. The behavior of enzymes in more complex systems is discussed, and the intact cell is thoroughy examined. Fundamentals of Enzymology also provides complete information on enzyme turnover in vivo, clinical aspects of enzymes, and enzyme technology. This new edition pays close attention to new applications of recombinant-DNA technology, and includes the latest information on the production of enzymes, the determination of amin-acid sequences, and the analysis of enzyme structure and function using site-directed mutagenesis |
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Contents
The purification of enzymes | 17 |
References | 52 |
The structure of enzymes | 56 |
Copyright | |
11 other sections not shown
Common terms and phrases
6-phosphate activity addition amino acids amount aspartate binding Biochem Biochemistry biol bonds catalysed cell cent changes Chapter Chem chemical chromatography coli complex concentration constant containing cytosol degradation dehydrogenase derived described detailed determined discussed dissociation domain effects enzyme equilibrium example experiments fragments give given glucose glycogen hydrolysis important increase inhibition inhibitor interactions involved isolated kinase kinetics known lactate ligand liver measured mechanism membrane methods mitochondria modification molecules multienzyme muscle NADH Nature obtained occur organisms particular pathway peptide phosphate phosphorylase plot polypeptide chain possible present procedure proteases protein purification pyruvate reaction reference regulation respectively Section separation sequence shown side-chain similar solution specificity step structure studies substrate subunits synthase synthesis Table techniques tissue values various