Fundamentals of enzymology
Now in a new edition, this book provides an up-to-date account of the field of enzymology. it describes the action of enzymes in systems of increasing complexity, and shows how these properties can be best utilized, both clinically and technically. The reader will find in-depth coverage purified enzymes, including full treatment of purification methods, structure, kinetics, mechanism of action, and the control of enzyme activity. The behavior of enzymes in more complex systems is discussed, and the intact cell is thoroughy examined. Fundamentals of Enzymology also provides complete information on enzyme turnover in vivo, clinical aspects of enzymes, and enzyme technology. This new edition pays close attention to new applications of recombinant-DNA technology, and includes the latest information on the production of enzymes, the determination of amin-acid sequences, and the analysis of enzyme structure and function using site-directed mutagenesis.
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The mechanism of enzyme action
The purification of enzymes
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6-phosphate adenosinetriphosphatase adenylate adenylate kinase affinity amide bond amino acids amino-acid sequence arginine aspartate atoms binding Biochemistry biol Biophys carbamoyl phosphate catalysed catalytic activity cell cent changes Chapter Chem chromatography chymotrypsin coli complex concentration containing covalent cysteine cytochrome cytosol D-glucose degradation determined dissociation disulphide bonds electrophoresis elution enzyme activity Enzymes 3rd edn equilibrium example fatty-acid fraction fragments fructose-bisphosphate aldolase glucose glutamate glycogen hexokinase hydrogen bonds hydrolysis inactivation inhibition inhibitor interactions intracellular involved isoenzymes isolated isomerase kinase kinetics lactate dehydrogenase ligand liver malate mechanism membrane metabolic Methods Enzymol mitochondria molecules multienzyme protein muscle N-terminal NAD+ NADH nucleotides nucleotidyltransferase occur ornithine orthophosphate oxidation pathway peptide phosphatase phosphorylase polypeptide polypeptide chain procedure proteases purification pyruvate dehydrogenase reaction catalysed Section solution specificity step studies substrate subunits synthase synthesis synthetase technique three-dimensional structure tissue Trends Biochem trypsin tryptophan X-ray X-ray crystallography