GB 4789.10-2016 Translated English of Chinese Standard. GB4789.10-2016: National food safety standards - Food microbiological examination - Staphylococcus aureushttps://www.chinesestandard.net, 2017 M11 17 - 24 pages This Standard specifies the examination method for Staphylococcus aureus in foods. Method I of this Standard applies to the qualitative examination of Staphylococcus aureus in foods; Method II applies to the counting of Staphylococcus aureus in foods with high Staphylococcus aureus content; Method III applies to the counting of Staphylococcus aureus in foods with low Staphylococcus aureus content. |
Contents
Examination procedure | 8 |
Result calculation | 10 |
Report | 11 |
Examination procedure | 12 |
Result and report | 13 |
| 14 | |
Annex B Staphylococcal enterotoxin detection | 19 |
Retrieval table for most probable number MPN of staphylococcus aureus | 24 |
Common terms and phrases
20.0 g Distilled 25 g mL ADJUST the pH aureus in foods aureus MPN counting AUTOCLAVE at 121 Baird-Parker plate blood plate calculated as equation CENTRIFUGE chloride 5.0 g clear band containing 225 mL counted and calculated critical value Crystal violet culture at 36 dilution plate distilled water Distilled water 1000 E-type Staphylococcal enterotoxin enzyme Erlenmeyer flask ethanol Examination procedure g of Na2HPO4 heptane identification test INCUBATE iodine microporous strip mL of distilled mL of sample negative quality controls number of colonies number of Staphylococcus number of typical nutrient agar slant Peptone phosphate buffer pipette plasma coagulase test Potassium dihydrogen phosphate Potassium iodide procedure of Staphylococcus r/min Rabbit plasma reagents room temperature Safranin sample solution select sample homogenates Sodium chloride 5.0 sodium chloride broth sodium citrate sodium hydroxide sodium hypochlorite Staphylococcus aureus Staphylococcus aureus colonies Staphylococcus aureus plate test tube total number transparent hemolytic circle typical colonies