GB 4789.12-2016 Translated English of Chinese Standard. GB4789.12-2016: National Food Safety Standard - Food microbiological examination - Clostridium botulinum and botulinum toxin testhttps://www.chinesestandard.net, 2017 M11 4 - 20 pages This standard specifies the test method for the clostridium botulinum and botulinum toxin in food. This standard applies to the test of the clostridium botulinum and botulinum toxin in food. |
Contents
| 3 | |
Scope | 4 |
Medium and reagents | 5 |
Test procedures | 6 |
Operating procedures | 7 |
Result report | 15 |
Appendix A Medium and reagent | 16 |
Common terms and phrases
ADJUST the pH agar plate amplification band anaerobic culture botulinum and botulinum botulinum neurotoxin botulinum toxin diagnosis botulinum toxin gene botulinum toxin poisoning botulinum toxin test capillary electrophoresis centrifuge tube clostridium botulinum confirmation test Crystal violet culture at 35 DISSOLVE the ingredients distilled water DNA template Egg yolk agar electrophoresis enrichment bacteria solution enrichment culture g centrifugation GB/T gelatin phosphate buffer Glucose Gram stain high pressure steam iodine lysozyme meat culture medium meat residue Medium and reagent mice onset MIX it uniformly MLD/mL mol/L sodium hydroxide negative control Nucleic acid PCR amplification products Peptone phosphate buffer solution PIPETTE Potassium iodide Preparation method DISSOLVE pressure steam sterilization primer refrigerator Safranine sample homogenate SHAKE it uniformly shows the amplification single type diagnosis spectrophotometer subject to 121 subject to anaerobic supernatant TBE buffer test solution toxicity toxin diagnosis serum Trypsin solution tryptone type diagnosis serum water to wash yolk agar medium