GB 4789.34-2016: Translated English of Chinese Standard. GB4789.34-2016: National Food Safety Standard - Food Microbiological Examination - Examination of Bifidobacterium
https://www.chinesestandard.net, 2017年11月4日 - 16页
This Standard specifies the identification and counting method for Bifidobacterium. This Standard applies to the identification and counting of pure bacteria strain of Bifidobacterium. This Standard applies to the identification of bacteria when the food only contains single Bifidobacterium. This Standard applies to the counting when the food only contains Bifidobacterium, i.e., the food may contain one or more different Bifidobacterium species.
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1:10 sample homogenizing 2min 3.0mL of Bifidobacterium acetic acid standard acetone acetonitrile acid or lactic acid standard solution Adding distilled water Agar powder analytically pure appropriate dilution autoclave at 121°C average number beat 1min Bifidobacterium agar plate Bifidobacterium culture medium Biochemical identification blank culture medium calibrate to pH catalase chloroform Colony counting culture at 36°C cysteine solution dihydrogen phosphate diluent disaccharide dissolve by heating extended to 72h flake Food sample g Yeast hemoglobin solution homogeneous bag containing homogenizer to beat homogenizing at 8000r/min inoculate on Bifidobacterium lactic acid standard Methanol milliliter mL of water mol/mL sodium hydroxide multiplied number of colonies number of plate operation steps peak area Peptone Perform anaerobic culture phenolphthalein phosphate physiological saline pipette plate colonies Preparation PYG liquid medium saline sample homogenizing solution Sample processing slapping homogenizer sodium chloride sodium hydroxide solution strains are positive sulfuric acid titration total number vitamin K1 solution water to dilute