GB 4789.35-2010 Translated English of Chinese Standard. GB4789.35-2010: National food safety standard - Food microbiological examination: Lactic acid bacteria
https://www.chinesestandard.net, 13. feb. 2015 - 15 sider
This standard specifies test methods of lactic acid bacteria in foods containing lactic acid bacteria. This standard applies to the test of lactic acid bacteria in foods containing lactic acid bacteria.
Culture medium and reagents
Results and report
0.1mL of sample 1:10 sample uniform 2~3 continuous suitable 2~3 suitable dilutions 225mL of saline absorb 0.1mL Adjust pH ammonium oxalate anaerobic method appendix A.3 appropriate counting range Autoclave average colony number bacteria in foods bromocresol purple alcohol Carbohydrates Cellobiose coating in 15min coating respectively Colonies count containing 225mL continuous suitable dilutions Crystal violet dilution to plate Erlenmeyer flasks Esculin ethanol fermentation tube Finish the process flaky colonies fluid to place GB/T Glucose Gram dye Heat to dissolve improved Li-mupirocin Inoculate Inspection procedure L-shaped bar lactic acid bacteria Lactobacillus count Lactose Li-mupirocin MRS culture Li-mupirocin stock solution liquid sample lowest dilution-times Maltose MC agar plates MC culture medium method to cultivate micropipette neutral red Peptone 5.0g plate coating plates for coating Preparation method Add process from dilution rounding rules sample uniform fluid select 2~3 continuous Select 2~3 suitable sterile homogeneous Streptococcus thermophilus count Sucrose surface coating total number unit to report Yeast