GB 4789.35-2010: Translated English of Chinese Standard. GB4789.35-2010: National food safety standard - Food microbiological examination: Lactic acid bacteria
https://www.chinesestandard.net, 13 thg 2, 2015 - 15 trang
This standard specifies test methods of lactic acid bacteria in foods containing lactic acid bacteria. This standard applies to the test of lactic acid bacteria in foods containing lactic acid bacteria.
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Culture medium and reagents
Results and report
0.1mL of sample 0.22µm microporous membrane 1:10 sample uniform 1.4mL Distilled water 2~3 continuous suitable 2~3 suitable dilutions 225mL of saline absorb 0.1mL Adjust pH alcohol solution 1.4mL ammonium oxalate anaerobic method Apparatuses and materials appendix A.3 Autoclave average colony number bacteria in foods Bifidobacterium count Carbohydrates Cellobiose coating in 15min coating respectively Colonies count containing 225mL continuous suitable dilutions Crystal violet dilution to plate Erlenmeyer flasks Esculin ethanol fermentation tube Finish the process flaky colonies fluid to place foods containing lactic GB/T Glucose Gram dye Heat to dissolve improved Li-mupirocin Inoculate Inspection procedure L-shaped bar lactic acid bacteria Lactobacillus count Lactose liquid sample Maltose MC agar plates method to cultivate micropipette neutral red number of lactic Peptone 5.0g plate coating plates for coating Preparation method Add process from dilution rounding rules sample uniform fluid select 2~3 continuous Select 2~3 suitable sterile homogeneous Sucrose surface coating total number unit to report Yeast