GB 4789.36-2016: Translated English of Chinese Standard. GB4789.36-2016: National Food Safety Standard - Microbiological Examination of Food Hygiene Examination of Escherichia Coli O157:H7/NM
https://www.chinesestandard.net, Nov 4, 2017 - Technology & Engineering - 17 pages
This Standard specifies the test method of Escherichia coli o157:h7/nm in foods. This Standard applies to the test of Escherichia coli o157:h7/nm in foods.
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Annex A Culture Media and Reagents
Common terms and phrases
225 ml mEC+n Add dropwise apparatus and materials Beads Capture Method Beef extract calibrate the pH cefixime chloride 5.0 g chromogenic agar plate coli O157 chromogenic coli O157 immunomagnetic Conduct autoclaved sterilization crystal violet CT-SMAC plate culture for 18 Dissolve all ingredients Distilled water Escherichia coli O157 Escherichia coli O157:H7 Escherichia Coli O157:H7/NM ethyl alcohol Examination of Escherichia Examination of Food fluorescence Food Hygiene g Distilled g or 25 g Sodium Gram staining Gram-negative homogeneous bag containing Immunomagnetic Beads Capture immunomagnetic beads suspension load separately Lysine decarboxylase magnetic beads magnetic plate frame Method of Escherichia Microbiological Examination microcentrifuge tube ml having graduates ml of mEC+n neutral red novobiocin nutrient agar plate O157 chromogenic agar O157 immunomagnetic beads oxidase test oxidase-negative pipettes plate and Escherichia potassium tellurite Preparation Dissolve product manuals Safranine Shiga toxin Sodium chloride Sorbitol specimen mixer stain solution sterilization for 15 supernatant suspicious colonies tellurite Test Procedures