GB 4789.40-2016: Translated English of Chinese Standard. GB4789.40-2016: National Food Safety Standard - Food Microbiological Examination - Examination of Cronobacter (Enterobacter Sakazakii)
https://www.chinesestandard.net, 04/11/2017 - 14 páginas
This Standard specifies the examination method for Cronobacter in food. This Standard applies to the examination of Cronobacter in infant formula, milk and dairy products and their raw materials.
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0.015 g Distilled 1.0 g Bromocresol 3.0 g Glucose 5.0 g Yeast add into 900 adjust pH Agar amygdalin Autoclave at 121°C Bromocresol purple 0.015 Buffer peptone water chloride 5.0 g Culture at 30°C Culture at 36°C D-sucrose decarboxylase test dissolve the components Distilled water 1000 Examination of Cronobacter Experimental method Inoculate filter paper Food Microbiological Examination g Dipotassium phosphate g mL g Potassium dihydrogen g Sodium g Yeast extract Glucose 1.0 Heat and dissolve Inoculate the culture L-lysine decarboxylase medium L-ornithine decarboxylase medium lauryl sulfate tryptose liquid level liquid medium medium is purple mL of BPW mLST-Vm mLST-Vm broth Modified lauryl sulfate monohydrochloride 5.0 National Food Safety negative is yellow Observe the results Oxidase reagent oxidase test phosphate 1.0 phosphate 2.75 g pipette Potassium dihydrogen phosphate preheated to 44°C Preparation method Heat Sodium chloride 5.0 Sub-packaging 5 mL test is positive transfer 1 mL Tryptone tube vancomycin solution Yeast extract 3.0