GB 4789.41-2016: Translated English of Chinese Standard. GB4789.41-2016: National food safety standards - Microbiological Examination of Food Hygiene - Examination of Enterobacteriaceae
https://www.chinesestandard.net, 2017 M11 17 - 18 pages
This Standard specifies the method for the test of Enterobacteriaceae in foods. The first method of this Standard applies to the bacterial counts of Enterobacteriaceae in the foods which has a high content of Enterobacteriaceae; and the second method applies to the bacterial counts of Enterobacteriaceae in the foods which has a low content of Enterobacteriaceae.
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Annex A Examples for Calculation of Results
Annex B Culture Media and Reagents
Retrieval Table of Enterobacteriaceae Most Probable Numbers MPNs
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1:1000 second dilution 12.0 g Distilled adjust the pH appropriate continuous dilution Bile salt chloride 5.0 g colony count/CFU 1:100 colony count/CFU Colony colony counting plates conduct autoclaved sterilization confirmation test/CFU Confirmed confirmed colony count Confirmed colony count/CFU confirmed Enterobacteriaceae colony continuous dilution degrees count of Enterobacteriaceae count/CFU Colony count Crystal violet degree Typical colony degrees include appropriate dilution degrees include Disodium phosphate Dissolve the ingredients Distilled water EE broth Enterobacteriaceae confirmed Enterobacteriaceae Plate Count ethyl alcohol extract 3.0 glucose agar Glucose fermentation test Gram stain Gram-negative heat to boiling homogeneous specimen solutions include appropriate typical inoculating loop iodine liquid specimen load separately minimum dilution factor ml of BPW Neutral red number is rounded oxidase test Potassium dihydrogen phosphate Potassium iodide Preparation Dissolve Result of Enterobacteriaceae Safranine second dilution degree specimen homogeneous solutions sterilization for 15 Test Procedures test tubes test/CFU Confirmed colony Typical colony count/CFU typical colony counts