GB 4789.43-2016: Translated English of Chinese Standard. GB 4789.43-2016: National Food Safety Standard - Food microbiological examination - Microbial source enzyme preparation antibacterial activity determination
https://www.chinesestandard.net, 2017/08/11 - 10 ページ
This Standard specifies the method for the determination of antibacterial activity of microbial source enzyme preparation. This Standard is applicable to the determination of antibacterial activity of enzyme preparation produced by microorganism.
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1°C constant temperature 10-5 dilution solution 6.4 Preparation 9 mL agar Antimicrobial activity report bacillus cereus bacillus circulans ATCC bacteria suspension solution bacteria suspension stock bath box containing 100 µL containing test enzyme control paper containing cooled to 46°C Cultivate at 36°C Cultivate at 36°C±1°C Determination of concentration determination Report Figure Distilled water Enzyme preparation paper enzyme preparation sample enzyme preparation solution escherichia coli gradient dilution insulation before bacteria mL of TSB negative control paper negative control plate paper containing ciprofloxacin paper containing test paper to staphylococcus petri dish pieces of paper plate after solidification Preparation method Pipet prepared in 6.1 prepared to 10-4 pyogenes ATCC 12344 Respectively pick respectively prepared serratia marcescens Sodium chloride solidified solution prepared staphylococcus aureus sterile paper sterile plate sterile saline stock solution Respectively streptococcus pyogenes ATCC suspension solution pavement suspension stock solution test bacteria suspension test enzyme preparation TSA medium TSA plate TSB broth