GB 5009.154-2016 Translated English of Chinese Standard. GB5009.154-2016: National food safety standard - Determination of Vitamin B6 in Foodshttps://www.chinesestandard.net, 2018 M07 21 - 19 pages This Standard specifies methods of determining vitamin B6 in foods. In this Standard, Method I is high-performance liquid chromatography, which is applicable to the determination of foods that contain vitamin B6; Method II is microbial method, which is applicable to the determination of vitamin B6 in all kinds of food. |
Contents
| 3 | |
Scope | 4 |
Instruments and Equipment | 6 |
Expression of Analysis Results | 8 |
Precision | 9 |
Instruments and Equipment | 11 |
Expression of Analysis Results | 13 |
Precision | 14 |
Appendix A Method of Concentration Correction of Vitamin B6 in Each Component of Standard Solution | 15 |
Appendix B Liquid Chromatogram of Vitamin B6 | 17 |
Medium Component and Preparation Method | 18 |
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Common terms and phrases
0.1 mol/L hydrochloric 150 mL conical accordance with Formula accurate to 0.01 accurately weigh-take add water adjust to pH amylase arithmetic mean autoclave B6 in Foods Bromo cresol green broth agar medium calculated in accordance chromatograph component pyridoxine concentration constant volume content of vitamin culture under 30 Determination of Vitamin distilled water expressed in mg/100 GB/T Glacial acetic acid high-performance liquid chromatography hydrochloric acid solution inoculate later usage mL conical flask mL of pyridoxine mol/L hydrochloric acid monthly-reserved strain national-level certificate pyridoxal and pyridoxamine Pyridoxine hydrochloride Pyridoxine standard stock pyridoxine Y medium remain valid Saccharomyces carlsbergensis sample solution sample tube seed culture medium Sodium chloride Sodium hydroxide solution solution 0.1 mol/L solution to dissolve spectrophotometer standard curve line standard stock solution standard substance sterilization under 121 sulfuric acid Table A.1 Triethylamine volume of 100 volumetric flask Vortex mixer water to dilute water to dissolve YM broth agar YM broth medium