GB 5009.181-2016: Translated English of Chinese Standard. (GB5009.181-2016): National food safety standard - Determination of malondialdehyde in food
https://www.chinesestandard.net, 2018 M12 8 - 11 pages
This Standard specifies the method for determination of malondialdehyde in food. Method 1 of this Standard is applicable to the determination of malondialdehyde in food. Method 2 of this Standard is applicable to the determination of malondialdehyde in animal and vegetable fats and oils.
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1,1,3,3-tetraethoxypropane also known 90 C water accurate to 0.01 accurately PIPETTE 0.10 Accurately TRANSFER 0.315 acid mixture 3.2.2 ADD 50 mL ADD trichloroacetic acid Ammonium acetate Aqueous solution arithmetic mean conical flask content of malondialdehyde COOL to room determination of malondialdehyde diamine tetraacetic acid dilute to 100 DILUTE to volume dissolved in water double-layer quantitative slow Ethylene diamine tetraacetic filtrate is standby high performance liquid independent determination results liquid chromatography malondialdehyde 100 malondialdehyde in food milligrams milliliter mixture to dilute mL colorimetric tube mL of standard mL of trichloroacetic mL volumetric flask mol/L obtained under repeated oscillator to shake performance liquid chromatography PIPETTE 1.0 mL quantitation is 0.10 Reagents repeated conditions room temperature series solution respectively solution of malondialdehyde solution of thiobarbituric Spectrophotometry standard curve standard series solution standard solutions Standard stock solution Standard use solution tetraacetic acid C10H14N2Na2O8 Thermostatic oscillator thiobarbituric acid TBA trichloroacetic acid mixture water to dissolve