GB 5009.211-2014 Translated English of Chinese Standard. GB5009.211-2014: National food safety standard - Determination of folates in foods
https://www.chinesestandard.net, 2016 M08 26 - 17 pages
This Standard specifies the method for determination of folates in foods. This Standard applies to the determination of folates in foods.
Apparatuses and instruments
Analytical steps all operations are conducted in dark
Appendix A Preparation methods for folates determination medium
1°C incubator 100mL of water 4°C refrigerator accordance with Clause accordance with equation activated carbon ADD water Adenine agar medium arithmetic mean AUTOCLAVE for 15min B-salt solution casein content in sample control tube Determination of folates Dipotassium phosphate Disodium hydrogen phosphate dissolve and dilute drops of toluene enzyme blank solution Enzyme extraction method folate content folate determination medium folates in foods folates standard stock guanine hundred grams hydrochloric acid hydrochloric acid solution incubator for 20h Lactobacillus casei lactobacillus rhamnosus Manganese sulfate microgram per hundred milliliter ng/mL MIX it uniformly nanograms per milliliter Polysorbate 80 Potassium dihydrogen phosphate preserved in 2°C quantification limit respectively WEIGH Sample and enzyme sample dilution sample extract sample series tube series test tubes Sodium acetate trihydrate sodium hydroxide sodium hydroxide ethanol Sodium hydroxide solution standard series test standard stock solution stock strain STUFF with tampon toluene transmittance or absorbance unit of nanograms uracil volumetric flask water to dissolve