GB 5009.22-2016 Translated English of Chinese Standard. (GB5009.22-2016): National food safety standard - B-group and G-group aflatoxins in foodshttps://www.chinesestandard.net, 2018年11月16日 - 59 pages This standard specifies the method for determination of aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2 (hereinafter referred to as AFT B1, AFT B2, AFT G1 and AFT G2) in food. The first method of this standard is the isotope dilution liquid chromatographytandem mass spectrometry, which is suitable for the determination of AFT B1, AFT B2, AFT G1 and AFT G2 in cereals and their products, beans and their products, nuts and seeds, fats and their products, seasonings, infant formula and infant complementary foods. |
Contents
Expression of analytical results | 31 |
Precision | 32 |
Principle | 33 |
Analytical procedures | 34 |
Precision | 35 |
Principle | 36 |
Instruments and equipment | 38 |
Precision | 46 |
Analytical procedures | 19 |
Expression of analytical results | 21 |
Precision | 22 |
Principle | 23 |
Instruments and equipment | 25 |
Analytical procedures | 27 |
Appendix A Calibration method of standard concentration of AFT B1 AFT B2 AFT G1 and AFT G2 | 47 |
Appendix B Method of verification of immunoaffinity column | 49 |
Tandem mass spectrometry | 50 |
Liquid chromatogram | 55 |
Appendix E Determination method of quality of enzymelinked immunosorbent kit | 59 |
Common terms and phrases
50 mL centrifuge accurate to 0.01 Accurately PIPETTE acetonitrile aflatoxin B1 AFT B1 standard AFT G2 bromine certified and awarded chloroform concentration content of AFT derivatization derivatization of four detection limit Determination of aflatoxin dilution disodium hydrogen phosphate elution evaporating dish four aflatoxins g of specimen G1 and AFT GB/T glass-fiber filter paper homogenized by homogenizer immunoaffinity column iodine ion scan diagram isotope internal standard limit of AFT limit of quantification liquid chromatography methanol methanol-water solution 70 microporous filter milliliter MIX it uniformly mL centrifuge tube mL of acetonitrile mL of water mL/min nationally certified oscillator or shaker PIPETTE point-drop quantification of AFT r/min reagent reference conditions separatory funnel shaker to oscillate shown in Figure soy sauce specimen accurate standard stock standard stock solution standard substance certificate standard working solution supernatant supernatant to prepare TAKE the supernatant thin-layer plate trifluoroacetic acid ultrasonic/vortex oscillator volume reach volumetric flask wavelength