GB 5009.255-2016: Translated English of Chinese Standard. GB5009.255-2016: National food safety standard -- Determination of fructan in foods
https://www.chinesestandard.net, 2017 M04 29 - 19 pages
This standard specifies the determination of fructan content in food by ion chromatography. This standard applies to the determination of oligofructose, polyfructose or inulin content separately added in milk and dairy products, infants and young children formula, infants and young children cereals, solid beverages and formulated wines.
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Expression of analysis results
Annex A Fructose standard solution milk powder sample chromatogram
Annex B Method for determination of enzyme activity
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10mL graduated test 3,5-dinitrosalicylic acid solution 50mL beaker Accurately weigh acetate buffer solution boiling water bath chromatogram Reference conditions concentration of fructose constant temperature water Cool to room dilute to 1000 dinitrosalicylic acid Eluents enzyme activity fructan content Fructose standard solution Fructose standard substance g of sodium Glacial acetic acid Glucose and fructose hydrolyze inulin maleate buffer solution Maleic acid mass concentration mg/L Milk powder sample milligram milliliters mL mL of hot nearest of 0.01 oligofructose Phenol Pipette potassium sodium tartrate powder sample chromatogram Prepare when required r/min reagent blank reducing sugar room temperature sample solution set volume sodium acetate buffer sodium borohydride sodium hydroxide solution sodium maleate buffer Sodium potassium tartrate Sodium sulfite solution 200 mmol/L specimen standard curve standard solution chromatogram Store at 4°C Sucrase activity temperature water bath testing enzyme water and dilute water bath shaker water to dilute water to dissolve wavelength of 540