GB 5009.259-2016: Translated English of Chinese Standard. GB5009.259-2016: National food safety standard -- Determination of biotin in foods
https://www.chinesestandard.net, 2017 M04 29 - 13 pages
This standard specifies the method for determination of biotin in food. This standard applies to the determination of biotin in food.
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Instruments and equipment
Preparation and preservation of strains
Appendix A Medium and reagent
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100 mL volumetric ABSORB accordance with Appendix Accurately PIPETTE 1.00 Accurately WEIGH ADD water ADJUST the pH arithmetic mean biotin content biotin determination culture Biotin determination medium biotin in food buffer solution CENTRIFUGE chloride solution 0.85 citric acid constant temperature incubator determination culture medium determination of biotin different test tubes ethanol solution 50 food safety standard g of glucose g of sodium Hydrochloric acid incubator at 37 independent determinations obtained inoculum Lactobacillus agar medium light transmittance mark V1 MIX it uniformly mL of biotin mL of water mL volumetric flask mol/L National food safety obtained under repeatability Papain PIPETTE 1.00 mL potassium dihydrogen phosphate prepared in accordance PRESERVE Reagents reserve strain sample extract sample solution sodium chloride sodium chloride solution sodium hydroxide sodium hydroxide solution solution 10 ng/mL standard curve tube standard series tubes standard substance standard working solution STERILIZE Sulfuric acid TRANSFER volume reach yeast extract Ág/mL