GB 5009.274-2016: Translated English of Chinese Standard. GB 5009.274-2016: National Food Safety Standard - Determination of Ciguatoxin in Aquatic ProductsThis Standard specifies methods for the determination of ciguatoxin in aquatic products. This Standard applies to the determination of ciguatoxin in edible parts of aquatic products. |
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Contents
3 | |
Scope | 4 |
Instruments and apparatuses | 5 |
Analysis steps | 6 |
Description of the analysis result | 7 |
Others | 8 |
Reagents and materials | 9 |
Instruments and apparatuses | 10 |
Description of the analysis result | 13 |
Precision | 14 |
Appendix A The relationship of time of death that is caused by ciguatoxin mouse unit | 15 |
Appendix B Table of the correction coefficient of mouse weight | 16 |
Multiple reaction monitoring chromatogram of ciguatoxin PCTX1 P CTX2 PCTX3 mixed standard solution | 17 |
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Common terms and phrases
according to Formula acetone Ammonium acetate solution appropriate amount aquatic product blank control group blank solution C18 solid phase caused by ciguatoxin Chloroform chromatographic pure chromatography-tandem mass spectrometer Ciguatoxin in Aquatic ciguatoxin P-CTX-1 coefficient of mouse Collision voltage concentration of ciguatoxin correction coefficient Determination of Ciguatoxin diethyl ether fix-volume formic acid group Each mouse intraperitoneally injected Liquid chromatography liquid chromatography-tandem mass median death method mice Mixed standard intermediate mL of methanol mL of n-hexane mL of water Mouse bioassay mouse unit mouse weight nitrogen to concentrate peak area phase extraction column Picric acid qualitative r/min reagents are analytical reference conditions relative abundance Rotary evaporate Sample extraction sample solution separatory funnel series working solution SN/T sodium hypochlorite solid phase extraction solution 10 mmol/L solvent-control group standard curve standard stock solution stored at 18°C Table A.1 Take the edible tandem mass spectrometry test group Tween 60-normal saline virulence of ciguatoxin volumetric flask