GB 5009.276-2016: Translated English of Chinese Standard. GB5009.276-2016: National Food Safety Standard - Determination of Glucono Delta-Lactone in Foods
https://www.chinesestandard.net, 2019年4月7日 - 12页
This Standard specifies methods for the determination of glucono delta-lactone in foods. Method 1 of this standard is applicable to the determination of glucono delta-lactone content in meat, meat products, soy products and beverages; method 2 of this standard is applicable to the determination of glucono delta-lactone content in foods.
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100 mL volumetric 50 mL beaker 6-phosphogluconate dehydrogenase 6-PGDH add 30 mL adenine dinucleotide phosphate adenosine triphosphate area of gluconic arithmetic mean content of glucono cover and mix D-(+)-gluconic acid Delta-Lactone in Foods determination of glucono filtrate as standby g of sample gluconic acid glucono delta-lactone content glucono delta-lactone standard high performance liquid Homogenizer Magnesium chloride measure the absorbance meat products milliliter mL of water mL volumetric flask Monopotassium phosphate monopotassium phosphate-phosphoric acid nicotinamide adenine dinucleotide peak area performance liquid chromatography Petroleum ether phosphate-phosphoric acid buffer Phosphoric acid potassium hydroxide solution put the filtrate quantitation-limit is 0.025 reagents remixed when activated ribulose 5-phosphate sample accurate Sample preparation sample solution series working solution Sodium bicarbonate solution 10 mg/mL solution 2 mol/L solution 50 mg/mL solution pH standard curve stored at 4°C test solution unit is mL water to dissolve water to fix-volume wavelength of 365 weigh 2 g