GB 5009.282-2020 Translated English of Chinese Standard (GB5009.282-2020, GB5009.282-2020): National food safety standard - Determination of 1-methylimidazole, 2-methylimidazole and 4-methylimidazole in foods
https://www.chinesestandard.net, 2022 M08 27 - 13 pages
This standard specifies the method for the determination of 1-methylimidazole, 2-methylimidazole, and 4-methylimidazole in food by liquid chromatography-tandem mass spectrometry. This standard applies to the determination of the content of 1-methylimidazole, 2-methylimidazole, and 4-methylimidazole in condensed milk, frozen drinks, jams, soy products, cocoa and chocolate products, candy, wheat flour products, biscuits, fillings and surface slurries, meat products, flavored syrups, condiments (soy sauce, vinegar, sauce and sauce products, cooking wine, compound seasoning), beverages, wine, jelly, and puffed food.
1-methylimidazole-D 4-methylimidazole 50 mL centrifuge accurate to 0.001 acetate solution 9+1 acetonitrile acid aqueous solution add 1 mL add 20 mL ammonia methanol solution ammonium acetate solution beverages brown volumetric flask CAS number certificate chocolate products chromatographic peak chromatographically pure component compound concentration determination dilute dissolve elute filter Flow formic acid aqueous g of uniformly heat homogeneous internal standard mixed isotope internal standard Keep liquid chromatograph mass spectrometry conditions Measure mixed standard mixed working solution mL brown volumetric mL centrifuge tube mL of ammonia mL of water months ng/mL obtained phase extraction column Pipette preparation purified later purity 98 qualitative ion Quantitative r/min ratio Reagents reference conditions Reference mass spectrometry respectively rinse sample solution sample the weight sauce sealed solid phase extraction specified speed standard series solution standard stock solutions standard substance certified supernatant Table temperature uniformly mixed sample voltage volume water bath Weigh 2 g