GB 5009.86-2016 Translated English of Chinese Standard. GB5009.86-2016: National Food Safety Standard - Determination of Ascorbic Acid in Foodshttps://www.chinesestandard.net, 2018 M02 11 - 18 pages This Standard specifies the high-performance liquid chromatography, fluorescence method, 2,6-dichloro-indophenol titration method for the determination of ascorbic acid in food. |
Contents
| 3 | |
Scope | 4 |
Principle | 5 |
Instruments and equipment | 6 |
Analysis steps | 7 |
Expression of analysis results | 8 |
Precision | 9 |
Principle | 10 |
Instruments and equipment | 11 |
Analysis steps | 12 |
Result calculation | 13 |
Precision | 14 |
Determination | 16 |
Precision | 17 |
Annex A L+ ascorbic acid D+ ascorbic acid standard chromatogram | 18 |
Common terms and phrases
2,6-dichloroindophenol solution consumed 20 g/L metaphosphoric 8°C from light accurately pipette Acid in Foods activated carbon add 5 mL amount of ascorbic arithmetic mean ascorbate standard solution ascorbic acid content ascorbic acid standard blank test chromatograph conversion factor dehydroascorbic acid dichloro-indophenol dilute to 100 diode array detector filtrate fluorescence intensity g/L metaphosphoric acid independent determinations obtained L-cysteine solution L(+)-dehydroascorbic acid mass concentration metaphosphoric acid solution methanol method mg/mL milligrams per hundred milliliter µg/mL milliliters mL nearest of 0.01 o-phenylenediamine obtained under repeatability oxalic acid solution oxidant phenylenediamine phosphoric acid pipette 10 mL Potassium dihydrogen phosphate purity 99 repeatability conditions Respectively and accurately sample blank solution sample mass sample solution set volume sodium acetate sodium bicarbonate solution or oxalic standard blank solution standard curve stored at 2°C sulfuric acid sulfuric acid solution test solution Thymol blue total amount Trisodium phosphate UV detector volume of 2,6-dichloroindophenol volumetric flask water and dilute