GB 5009.89-2016: Translated English of Chinese Standard. GB5009.89-2016: National food safety standard - Determination of niacin and nicotinamide in foods
https://www.chinesestandard.net, 2017年10月14日 - 20页
This Standard specifies the determination method for niacin and nicotinamide in foods. In this Standard, method I is microbiological method, which is applicable for the determination of the total content of niacin and nicotinamide in all types of foods including fortified foods using natural foods as the base. Method II is high performance liquid chromatography, which is applicable for the determination of acid and nicotinamide in fortified foods.
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0.1 mol/L hydrochloric 100-mL volumetric flask 20.0 g accurate according to equation accurate to 0.01 accurately WEIGH ADD water adjust the pH amylase arithmetic mean AUTOCLAVE calculated according content of niacin detection limit DILUTE with water Distilled water Erlenmeyer flask ethanol filter fortified foods g of sodium hydrochloric acid solution incubator at 36 inoculation solution isopropanol Lactobacillus agar medium Lactobacillus plantarum ATCC limit of quantification medium for niacin micrograms per gram mL of hydrochloric mL of niacin mL of water mol/L hydrochloric acid niacin and nicotinamide niacin content niacin determination niacin or nicotinamide nicotinamide standard stock optical density perchloric acid preparation of inoculation reagents relative standard deviation sample determination solution sample extract sample series tubes sample solution Sodium chloride sodium hydroxide solution solution 1 mol/L standard curve standard point standard series tubes standard stock solution stock strains sulfuric acid test tubes Ultrasonic oscillator water to dissolve wavelength WEIGH about 5.0