GB/T 14700-2018: Translated English of Chinese Standard. (GBT 14700-2018, GB/T14700-2018, GBT14700-2018): Determination of thiamine in feed
https://www.chinesestandard.net, 20. nov. 2018 - 15 sider
This Standard specifies two methods for the determination of thiamine B1 in feed, including fluorescence spectrophotometry and high performance liquid chromatography. Method I of this Standard applies to the determination of thiamine B1 in feed material, compound feed and concentrated feed. The quantitation limit of this method is 1 mg/kg (in case of the existence of any interfering substance which absorbs thiamine or influences thiamine fluorescence, this method is not applicable). Thiamine B1 determined in this Method includes the total of intrinsic and additive amounts.
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absorption column acetic acid solution acid solution 3.2.1 acidic potassium chloride add 25 mL ammonium chloride amylase arithmetical mean value B1 in feed B1 in sample B1 standard solution B1 standard stock brown bottle brown volumetric flask chromatographic compound feed compound premixed feed conical flask content is greater content of thiamine EDTA extracting solution feed whose thiamine filter membrane flask of 100 fluorescence intensity glacial acetic acid guaranteed reagent high performance liquid hydrochloric acid solution laboratory glassware membrane of 0.45 mL of thiamine mol/L Moving phase normal butanol potassium chloride solution potassium ferricyanide solution put on stopper quinine sulfate refrigerator at 2°C repeatable conditions results measured independently sample as specified sample solution sodium acetate sodium hydroxide specified in GB/T spectrophotometry standard stock solution standard working solution sulfate working solution sulfuric acid terms of thiamine thiamine B1 content Thiamine B1 determined thiamine B1 standard thiamine hydrochloride thiamine nitrate thiamine premixed feed triethylamine