GB/T 14701-2019: Translated English of Chinese Standard. (GBT 14701-2019, GB/T14701-2019, GBT14701-2019): Determination of Vitamin B2 in feeds
https://www.chinesestandard.net, 31. aug. 2019 - 14 sider
This Standard specifies the fluorescence spectrophotometry and highperformance liquid chromatography for the determination of vitamin B2 (namely riboflavin C17H20N10) in feeds. Method 1 of this Standard is applicable to the determination of vitamin B2 in animal and plant feed materials, compound feed and concentrated feed; the quantitation-limit is 0.25 mg/kg.
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100 mL brown accurate to 0.001 add 1 mL analytical arithmetic mean B2 stock solution brown conical flask brown volumetric flask Chapter chromatogram of vitamin Column concentrated feed cooling deionized water detection determination of vitamin determination values difference disodium hydrogen phosphate dissolve document EDTA excitation extract feeds whose vitamin Figure A.1 filter fluorescein fluorescence detector fluorescence intensity g accurate GB/T glacial acetic acid High-performance liquid chromatography independent determination results Injection volume intensity of test mark method mg accurate mg/kg mL brown conical mL brown volumetric mL of glacial mL of vitamin Mobile phase mol/L obtained under repeated particle Preparation refrigerator at 2°C repeated conditions riboflavin sample solution shake sodium dithionite sodium hydroxide solution solution contains standard chromatogram standard solution standard stock solution standard working solution test solution test tube triethylamine vitamin B2 content Vitamin B2 standard vitamin premix feed water to dilute wavelength weigh