What people are saying - Write a review
We haven't found any reviews in the usual places.
Analysis of Gels Following Electrophoresis
17 other sections not shown
Other editions - View all
2-mercaptoethanol acetic acid acrylamide agarose amino acid ammonium persulphate ampholytes Anal analysis antibody antigen apparatus autoradiography Biochem Biol bisacrylamide carrier constituents catholyte Chapter charge Chem Chrambach,A Coomassie blue crosslinked dehydrogenase described destaining detection detergent discontinuous buffer system electrode electrofocusing elution enzyme equilibration Ferguson plot Figure fluorography fractionation gel buffer gel concentration gel mixture gel slices gel surface gel tube glass gradient gels histones immunoelectrophoresis ionic strength isoelectric points isotachophoresis labelled linear loaded lower buffer reservoir membrane method migration mobility molecular weight moving boundary native proteins overlayered pH gradient phoresis plates polyacrylamide gel electrophoresis polymerisation polypeptides pore precipitation prepared procedure protease protein bands protein of interest radioactive reagent removed reservoir buffer resolution resolving gel riboflavin rod gel SDS-discontinuous SDS-PAGE separation slab gel solubilisation solution stacking gel staining steady-state stacking technique TEMED temperature thiol tion tracking dye Tris-HCl pH two-dimensional upper buffer reservoir urea usually voltage volume