Gel Electrophoresis of Proteins: A Practical ApproachB. D. Hames, David Rickwood |
Contents
Experimental Approach | 5 |
Apparatus | 18 |
Analysis of Gels Following Electrophoresis | 42 |
Copyright | |
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2-mercaptoethanol acetic acid acrylamide agarose ammonium persulphate ampholytes Anal analysis antibody antigen apparatus autoradiography Biochem Biol bisacrylamide carrier constituents catholyte Chapter charge Chem Chrambach Chrambach,A Coomassie blue crosslinked dehydrogenase described destaining detection detergent discontinuous buffer system electrode electrofocusing elution enzyme equilibration Ferguson plot Figure first-dimensional gel fluorography fractionation gel buffer gel concentration gel mixture gel slices gel surface gel tube glass plates gradient gels histones immunoelectrophoresis ionic strength isoelectric points isotachophoresis labelled linear loaded lower buffer reservoir membrane methanol method migration mobility molecular weight moving boundary native proteins overlayered pH gradient phoresis polyacrylamide gel electrophoresis polymerisation polypeptides pore precipitation prepared procedure protease protein bands protein of interest radioactive reagent removed reservoir buffer resolution resolving gel riboflavin rod gel SDS-discontinuous SDS-PAGE separation slab gel solubilisation solution stacking gel staining steady-state stacking technique TEMED temperature tion tracking dye two-dimensional upper buffer reservoir urea usually voltage volume