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plasmids and bacteriophages
Purification of DNA from living cells
Manipulation of purified DNA
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amino acid ampicillin amplified analysis animal antisense bacterial cell bacteriophage bacterium base-pairing Biotechnology blunt-ended BRCA1 carried cDNA cell extract centrifugation chromosome cleave cloned gene cloning experiment cloning vectors coded colonies containing control sequence copy number cule culture deletion digestion disease DNA fragment DNA polymerase DNA sequencing EcoRI enzyme example expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell hybridization hybridization probing identified infection cycle introns labelled lacZ ligase ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms P-galactosidase PCR product phage phage DNA phage particles plaques plasmid plasmid DNA polylinker polynucleotide primers probe problem promoter pUC8 purified reaction recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction sites result RFLP segment single-stranded DNA sticky ends strand structure synthesis T-DNA techniques template tion transcription transformed types virus viruses vitro yeast