Gene Cloning and Expression Technologies

Front Cover
Michael P. Weiner, Quinn Lu
BioTechniques Press, 2002 - Science - 603 pages

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Contents

Introduction
1
Electronic Cloning
7
Gene Finding in Eukaryotes
27
Novel Gene Discovery by In Silico EST MiningA Versatile
45
Prediction of Protein Cellular Attributes
57
DNA Engineering for
87
Mammalian Expression Systems
115
Monoclonal Antibody Expression in Mammalian Cells
143
Use of GPCRGa Fusion Proteins for the Analysis
305
Transient Production of HighTiter Retroviral Vectors
327
Generation of Recombinant Adenovirus Vectors by a Direct
341
IN VITRO GENE EXPRESSION TECHNOLOGIES
357
Prokaryotic Systems for In Vitro Expression
391
Ribosome Display Technology
413
Protein Affinity Purification and Analysis Technologies
445
GENE FUNCTION ANALYSIS GENOMICS
459

Recombinant Expression and Gene Function Analysis in Drosopbila
163
Plant Cell Culture Expression Systems
179
S pombeYeast Expression System
195
Protein Expression in LForm Bacteria
213
Methods for In Vivo Selection of Soluble Protein Domains
227
The Use of NusA Fusion Proteins to Improve Recombinant Protein
239
Hydrophobic Tagging of Immunogens for Iscom Incorporation
251
The Avian Alternative
267
Bioinformatics and HighThroughput Protein Production
281
Selection of Highly Specific and Sensitive ssDNA PhotocrossLinking
477
Application of Ribozyme Technology
493
Screening PhageDisplayed Combinatorial Peptide Libraries
511
An AllLiquid Format for the Yeast TwoHybrid Assay
527
The Emerging Utility of Oligonucleotide Microarrays
537
Multiplexed Molecular Profiling Transcription Assay in ArrayPlates
549
Liquid Chromatographyl Mass Spectrometry Analysis
565
Index
583
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