Gene Isolation and Mapping Protocols
Humana Press, 1997 - Science - 318 pages
An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.
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John Valdes and Danilo A Tagle
Linkage Analysis of Genetic Disorders
Gene Ordering and Localization by Linkage Analysis
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2X SSC 3'-terminal exon Acad agarose gel alleles amplification beads blot Boehringer buffer cDNA clones cDNA library CEPH coding containing contig cosmid coverslip database denatured detection digestion disease dNTP electrophoresis ethanol ethidium bromide exon exon trapping filter FISH fluorescent fragments genetic linkage genetic map genomic DNA genomic sequences genomic target human genome identify incubate internal exon labeled ligation linkage analysis loci locus LOD score markers metaphase method mg/mL mMEDTA mouse mRNA Natl Note Nucleic Acids Res nucleotides PCR product PCR reaction pellet PFGE phage phenotype physical map plates plugs Preparation primers probe protein Protocols pulsed-field gel query radiation hybrid recombination fraction restriction enzyme Resuspend room temperature screening server signal slides somatic cell hybrids Southern blot splice subcloning supernatant Swiss-Prot target DNA techniques tion transcription Tris-HCl tube Unix vector wash yeast yeast artificial chromosome