Gene probes: a practical approach
Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. ACompanion volume, Gene Probes 1, covers preparation of the probes.
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absolute ethanol agarose gel aliquots allow analysis antibody antigens autoradiograph buffer cell line Centrifuge chromosomes clones colloidal gold colonies concentration containing coverslip deionized denaturation described in Protocol detection digestion diluted distilled water DNA fragments DNA probes DNA sequences dNTP EDTA embedding Equipment and reagents ethidium bromide Figure filter-bound formamide gel electrophoresis gene genomic DNA glomeruli Incubate labelling laboratory markers membrane method mg/ml microcentrifuge tube microscope mismatch mixture Molecular mRNA mutation NaCI Natl Acad nitrocellulose nuclease nucleic acid nucleotide nylon oligonucleotide PCR products pellet phage pipette plasmid plastic plate polymerase pre-hybridization prepared primer procedure protein reaction recombinant resin restriction enzyme Resuspend reverse transcription RNase room temperature samples screening sections Sigma signal single-stranded slides sodium acetate Southern blotting specific staining step sterile target TBE buffer technique template tissue transfected transfer Tris-HCI vector washing Whatman