Growth factors: a practical approach
IRL Press at Oxford University Press, 1993 - Literary Criticism - 251 pages
There has been a major expansion of interest in growth factors reflecting their very important role in the regulation of the proliferation and differentiation of specific cell types. A large number of growth factors have already been identified but this probably represents only a fraction of all those that exist. This book is a step-by-step guide to studying growth factors, from the initial search for and characterization of new activities, to sophisticated in vivo analysis and molecular biology approaches such as DNA cloning. It also covers the detection and study of growth inhibitory factors. Growth Factors: A Practical Approach is an invaluable practical guide for both those entering growth factor research for the first time and more experienced researchers. As well as covering a wide variety of techniques, this book includes a valuable appendix covering all known growth factors.
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Known growth factors
What to do with a new factor
RTPCR on human keratinocyte RNA
19 other sections not shown
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3H]thymidine acetate acid aliquots Amersham analysis antibody assay autoclaved autoradiography binding bio-assay Biol bombesin bone buffer cDNA cell lines cell proliferation cellular Centrifuge cloning coli column concentration conjugate containing culture cyclic AMP cytokines denaturing described in Protocol detection differentiation diluted dishes DMEM EDTA ELISA emulsion ethanol expression Figure fluorescent intensity fractions fusion gene growth factor activity hybridization IGF-II Incubate inhibition inhibitor insulin Interleukin keratinocytes labelled ligand MCF-7 cells medium method mg/ml mitogens molecular molecules mouse mRNA NaCl ng/ml non-specific oligonucleotide osteoblasts PDGF PDGF-B pellet peptide phage pipette plaques plasmid plate poly(A+ polypeptide preparation primer probe produced purification radioactive reaction receptor resuspend riboprobe Rinse room temperature sample sections sequence serum Sigma signalling pathways slides sodium solution specific staining sterile stimulation Swiss 3T3 cells TGF-p tissue transcription trypsin tubes vitro vivo Wash yeast