Guide to Electroporation and Electrofusion
Academic Press, Dec 11, 1991 - Science - 592 pages
Guide to Electroporation and Electrofusion is designed to cover all relevant topics pertaining to both electroporation and electrofusion. Divided into four major parts, the book covers fundamental aspects, as well as more advance aspects of the electroporation-electrofusion relationship.
The book first covers the basic principles and fundamentals by presenting the most recent theoretical and experimental studies from various fields, such as physics, chemistry, and biology. Next, the book tackles the applications of electroporation and electrofusion in biology, such as transferring, manipulating, and transforming genetic materials. In the third section, the book discusses experimental protocols to serve as a guide when performing experiments using electroporation and electrofusion. The final section discusses the instruments needed to effectively perform an experiment that involves electroporation and electrofusion.
This book will be of great used to both novice and advanced researchers whose work involves electroporation and electrofusion, as it provides comprehensive information regarding these topics.
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Acad acid activity alignment application assay bacteria Biol Biology Biopbyr blastomere capacitor Cell Biology cell fusion cell membrane cell types chamber Chernomordik chromosomal cloning coli concentration culture cuvette CV-l cytoplasmic dielectrophoresis Dimitrov effect efﬁciency eggs electric ﬁeld electric pulse electrical breakdown electrodes electrofusion electroinsertion electropermeabilization electroporation Electroporation and Electrofusion electrotransformation embryos enzymes erythrocyte erythrocyte ghosts expression ﬁeld strength ﬁrst ﬂuorescence frequency fusion yield gene transfer genetic glycophorin gram-positive hybridoma incubated induced Kinosita kV/cm lipid bilayer liposomes mammalian cells medium method modiﬁcation molecular molecules monoclonal antibodies mouse Natl Neumann nuclear transplantation nuclei observed oocytes optimal parameters permeabilized plant cells plant protoplasts plasmid plasmid DNA pollen poration pores potential procedure proteins protocols protoplasts rabbit recombination sample selection signiﬁcant somatic hybrids Sowers species speciﬁc suspension targeting technique Teissie tissue transfection transformation transient transmembrane Tsong uptake V/cm voltage Zimmermann