Guide to Electroporation and Electrofusion
Donald C. Chang, Bruce M. Chassy, James A. Saunders, Arthur E. Sowers
Academic Press, Dec 2, 2012 - Science - 569 pages
Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.
Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques.
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Acad acid Acta activity application assay bacteria Biochim Biol Biology Biophys blastomere capacitor Cell Biology cell fusion cell membrane cell types chamber Chernomordik chromosomal cloning coli concentration culture cuvette CV-1 cells cytoplasmic dielectrophoresis effect efficiency eggs electric field electric pulse electrical breakdown electrodes electrofusion electroinsertion electropermeabilization electroporation Electroporation and Electrofusion electrotransformation embryos enzymes erythrocyte erythrocyte ghosts expression field strength fluorescence frequency fusion yield gene transfer genetic glycophorin gram-positive hprt hybridoma incubated induced Kinosita kV/cm lipid bilayer liposomes mammalian cells medium method Microbiol molecular molecules monoclonal antibodies mouse Natl Neumann nuclear transplantation nuclei observed oocytes optimal parameters permeabilized plant cells plant protoplasts plasmid plasmid DNA pollen poration pores potential Proc procedure proteins protocols protoplasts rabbit recombination resealing sample selection somatic hybrids Sowers species strain suspension targeting technique Teissie tissue transfection transformation transient transmembrane Tsong uptake V/cm voltage Zimmermann