Haematopoietic and Lymphoid Cell Culture

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Margaret J. Dallman, Jonathan R. Lamb
Cambridge University Press, Jul 13, 2000 - Science - 188 pages
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This invaluable reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact hematopoietic and lymphoid cells, and their maintenance as primary cultures. The text provides technical information on the signals and mediators required for the differentiation and growth of these cells, and is designed for laboratory investigators with limited practical experience in cell culture. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and the culturing of murine thymic explants. Each chapter has been written by experts who have practical experience of the techniques discussed to provide tips for avoiding common pitfalls, and sharing insight into the fundamental questions in cell biology and immunology addressed using each cell culture model.

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Haematopoietic differentiation of embryonic stem cells
Dendritic cells
Murine thymic explant cultures
T cells
B lymphocytes
Monocytes and macrophages
NK cells and LAK cells
Mast cells and basophils

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Page 178 - Effect of IL-3 and stem cell factor on the appearance of human basophils and mast cells from CD34+ pluripotent progenitor cells. J Immunol 1992; 148:772-777.

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