Haematopoietic and Lymphoid Cell Culture
Margaret J. Dallman, Jonathan R. Lamb
Cambridge University Press, Jul 13, 2000 - Science - 188 pages
This invaluable reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact hematopoietic and lymphoid cells, and their maintenance as primary cultures. The text provides technical information on the signals and mediators required for the differentiation and growth of these cells, and is designed for laboratory investigators with limited practical experience in cell culture. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and the culturing of murine thymic explants. Each chapter has been written by experts who have practical experience of the techniques discussed to provide tips for avoiding common pitfalls, and sharing insight into the fundamental questions in cell biology and immunology addressed using each cell culture model.
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activity adherent analysis anti-mouse antibodies antigen assay Austyn basophils beads bone marrow bone marrow cultures buffer cell clones cell culture cell development cell lines cell suspension cell types cells and basophils cells/ml centrifuge chemokines clones culture medium cytokines defmed dendritic cells described diluted embryonal stem cells expression FACS feeder cells fetal liver filter flask flow cytometry functional gene giant cells GM-CSF growth factors haematopoietic Immunol incubate irradiated isolated lineage lymphocytes lymphoid lymphopoiesis macrophages magnetic markers mast cells mature method mice molecules monocytes mouse murine ng/ml NK cells non-adherent cells organ culture PBMC pellet peripheral blood Petri dish phenotype pipette plates populations pro-B cells procedure progenitors proliferation protocols purified reagents rearrangement receptor remove Resuspend the cells RPMI Sigma specific spleen staining sterile stromal cell supernatant surface thymocytes thymus thymus lobes thymus organ tissue culture transfer tube U/ml ug/ml vitro vivo Wash