Handbook of Biological Confocal Microscopy

Front Cover
James Pawley
Springer Science & Business Media, Aug 4, 2010 - Science - 985 pages
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Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.
 

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Contents

1 Foundations of Confocal Scanned Imaging in Light Microscop y
1
2 Fundamental Limits in Confocal Microscopy
20
3 Special Optical Elements
43
Digitizing Image Data
59
5 Laser Sources for Confocal Microscopy
80
6 NonLaser Light Sources for ThreeDimensional Microscopy
126
7 Objective Lenses for Confocal Microscopy
145
8 The Contrast Formation in Optical Microscopy
162
30 4Pi Microscopy
561
Stimulated Emission Depletion and Other Reversible Saturable Optical Fluorescence Transitions Microscopy Concepts
571
32 Mass Storage Display and Hard Copy
580
33 Coherent AntiStokes Raman Scattering Microscopy
595
34 Related Methods for ThreeDimensional Imaging
607
35 Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen
627
36 Practical Confocal Microscopy
650
37 Selective Plane Illumination Microscopy
672

9 The Intermediate Optical System of LaserScanning Confocal Microscopes
207
10 DiskScanning Confocal Microscopy
221
11 Measuring the Real Point Spread Function of High Numerical Aperture Microscope Objective Lenses
239
12 Photon Detectors for Confocal Microscopy
251
13 Structured Illumination Methods
265
14 Visualization Systems for MultiDimensional Microscopy Images
280
15 Automated ThreeDimensional Image Analysis Methods for Confocal Microscopy
316
Photophysics and Photochemistry
338
17 Practical Considerations in the Selection and Application of Fluorescent Probes
353
18 Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy
368
19 Confocal Microscopy of Living Cells
381
20 Aberrations in Confocal and MultiPhoton Fluorescence Microscopy Induced by Refractive Index Mismatch
404
21 Interaction of Light with Botanical Specimens
414
22 SignaltoNoise Ratio in Confocal Microscopes
442
23 Comparison of WidefieldDeconvolution and Confocal Microscopy for ThreeDimensional Imaging
453
24 Blind Deconvolution
468
25 Image Enhancement by Deconvolution
488
26 FiberOptics in Scanning Optical Microscopy
501
27 Fluorescence Lifetime Imaging in Scanning Microscopy
516
28 MultiPhoton Molecular ExcitationLaserScanning Microscopy
535
29 Multifocal MultiPhoton Microscopy
550
38 Cell Damage During MultiPhoton Microscopy
680
39 Photobleaching
690
40 Nonlinear Harmonic Generation Optical Microscopy
703
41 Imaging Brain Slices
722
42 Fluorescent Ion Measurement
736
43 Confocal and MultiPhoton Imaging of Living Embryos
746
44 Imaging Plant Cells
769
45 Practical Fluorescence Resonance Energy Transfer or Molecular Nanobioscopy of Living Cells
788
46 Automated Confocal Imaging and HighContent Screeningfor Cytomics
809
47 Automated Interpretation of Subcellular Location Patternsfrom ThreeDimensional Confocal Microscopy
818
48 Display and Presentation Software
829
Correlational Light Microscopy and Electron Microscopy
846
50 Databases for Two and ThreeDimensional Microscopical Images in Biology
861
51 Confocal Microscopy of Biofilms Spatiotemporal Approaches
870
52 Bibliography of Confocal Microscopy
889
Appendix 1 Practical Tips for TwoPhoton Microscopy
900
Appendix 2 Light Paths of the Current Commercial Confocal Light Microscopes Used in Biology
906
Appendix 3 More Than You Ever Really Wanted to Know About ChargeCoupled Devices
918
Index
933
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