Handbook of Biological Confocal Microscopy

Front Cover
James B. Pawley
Springer Science & Business Media, 1995 - Science - 632 pages
3 Reviews
New edition of a reference originally published in 1989 and revised in 1990. The present edition includes 37 chapters (15 updated from the first edition, 21 new ones, and an annotated bibliography), and is nearly three times as long as the original. In particular, it reflects an increased recognitio
 

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Contents

Foundations of Confocal Scanned Imaging in Light Microscopy
1
Fundamental Limits in Confocal Microscopy
19
Quantitative Fluorescence Confocal Laser Scanning Microscopy CLSM
39
The Pixilated Image
55
Laser Sources for Confocal Microscopy
69
NonLaser Light Sources
99
Objective Lenses for Confocal Microscopy
111
The Specimen Illumination Path and Its Effect on Image Quality
127
Lens Aberrations in Confocal Fluorescence Microscopy
347
RealTime Stereo 3D Confocal Microscopy
355
SignaltoNoise in Confocal Microscopes
363
Comparison of WideFieldDeconvolution and Confocal Microscopy for 3D Imaging
373
Light Microscopic Images Reconstructed by Maximum Likelihood Deconvolution
389
Direct View Confocal Imaging Systems Using a Slit Aperture
403
Two New HighResolution Confocal Fluorescence Microscopies 4Pi Theta with One and TwoPhoton Excitation
417
Optical Considerations at Ultraviolet Wavelengths in Confocal Microscopy
431

The Intermediate Optical System of LaserScanning Confocal Microscopes
139
Intermediate Optics in Nipkow Disk Microscopes
155
The Role of the Pinhole in Confocal Imaging System
167
Photon Detectors for Confocal Microscopy
183
The Collection Processing and Display of Digital ThreeDimensional Images of Biological Specimens
197
Visualization Systems for Multidimensional CLSM Images
211
Mapping and Measuring Surfaces Using Reflection Confocal Microscopy
255
Fluorophores for Confocal Microscopy
267
Image Contrast in Confocal Light Microscopy
281
Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy
311
Confocal Microscopy of Living Cells
327
TwoPhoton Molecular Excitation in LaserScanning Microscopy
445
VideoRate Confocal Microscopy
459
Confocal Microscopy with Transmitted Light
479
Fluorescence Lifetime Imaging a New Tool in Confocal Microscopy
491
Imaging Immunogold Labels with Confocal Microscopy
507
Fiberoptics in Confocal Microscopy
515
Comparison of Various Optical Sectioning Methods
525
Mass Storage and Hard Copy
535
Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen
549
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