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2-mercaptoethanol 50 per cent absolute ethanol absorption acetic acid acrolein acrolein—Schiff aldehyde alkaline amino acid amino acid residues amino groups arginine basic buffer pH Carnoy cent ethanol cent w/v changes of distilled clear in xylene collagen complex condition of material covalently cryostat cryostat sections cysteine dehydration Dewax sections dissolve 0.1 distilled water disulphide bridges DNFB dye binding electrostatic enzyme Eosin Feulgen—Schiff FITC Fixation conditions fixation mixture fixed smears fluorescence fluorochromes followed by rinsing formaldehyde formalin fume cupboard glutaraldehyde haematoxylin histidine histochemical histochemistry keratin light microscopy lysine maleimide ml of absolute molecules Naphthol Naphthol Yellow Nitro Red nucleic acids Orange G Paraffin sections Pararosanilin Pararosanilin SO2)—Schiff Phloxine Picrosirius Protein staining methods Rationale resin e.g. Euparal Result room temperature Schiff reagent sections and bring slides smears of isolated SO2)—Schiff reagent Specific reagents Staining procedure structure synthetic resin e.g. thiol thiol groups Thionin tissue tryptophan tyrosine xylene