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24 hours absolute alcohol alum anilin aqueous solution basic stains blood celloidin celloidin methods Celloidin sections cells chemical chloroform clarifier clean clear color connective tissue cool copper cover cover-glass cytoplasmic dehydrate desired dilute dissociator dissolve distilled water drop employed eosin ether-alcohol filter Fix tissue fixation fixer Flemming's fluid formalin Formula fuchsin acid gentian violet glacial acetic acid glycerin glycerin-jelly grams granules grms hardening hematoxylin Hermann's fluid histological imbed imbedding mass injection iodin isolation knife mercuric chlorid methyl methylene blue microscope microtome minutes mixture mordant mordantage mount in balsam Muller's fluid muscle myelinic necessary Neutral stains nitric acid orange G osmic acid paper paraffin paraffin method paraffin sections picric picro-fuchsin potassium dichromate precipitate preparation preservation remove rinse in water running water safranin salt solution saturated shellac silver nitrate slide sodium soluble specimen Stain sections stock solution thin tion toluene transfer usually wash xylene Zenker's fluid
Page 92 - A Note on the Use of Anise Oil in histological Methods with special Reference to its Value in cutting serial Sections on the freezing Microtome.
Page 49 - J % collodion (§ 556). In a minute or more the collodion displaces the xylene and penetrates the sections and forms a delicate veil over their free surface. No harm .is done by leaving the sections in the collodion a considerable time, but a minute or two is sufficient. The slide is removed, allowed to drain for half a minute, and then put into a jar of 67 % alcohol (fig.
Page 25 - ... clamp the block of paraffin in the holder of the microtome so that the tissue will be at the proper level for cutting. If a ribbon microtome is used, heat the holder and melt the end of the block upon it. Cool and place the holder in its place in the microtome. Use a very sharp, dry razor for cutting the sections. The sections are made with a rapid, straight cut as in planing. Do not try to section with a drawing cut...
Page 30 - The knife should, therefore, be set at an obliquity of 15 to 20° or less, so that half or more of the blade is used in cutting the section. Recall that in the paraffin method the knife is usually to be set at right angles to the direction of the cut, and the stroke is a rapid straight one. Trim away the surrounding celloidin mass leaving enough, however...
Page 25 - ... for trimming and arranging the block be observed, they will adhere and thus form a ribbon. If the room is too cold or the paraffin too hard, the sections will roll; if it is too warm, the sections will crush or be imperfect. If a microtome in which the knife is not fixed is used, make the sections with a rapid straight cut as in planing. Do not try to section with a drawing cut as used in celloidin sectioning.
Page 14 - Fluid. — Absolute alcohol 6 parts Chloroform 3 parts Glacial acetic acid 1 part The penetration of the reagent is very rapid.
Page 56 - The label should furnish at least the following information: EXAMPLE. (1) The number of the preparation, the thickness of the cover-glass and of the section.
Page 92 - Macallum, AB On the Nature of the Silver Reaction in Animal and Vegetable Tissues. Proc. Roy. Soc. Vol. LXXVI. 1905. p. 217.