Holographic Microscopy of Phase Microscopic Objects: Theory and Practice
World Scientific, 2011 - Electron microscopes - 108 pages
The book presents a clear and comprehensive review of the current status of the holographic microscopy with discussion of the positive and negative features of classical and holographic methods for solving the problem of three-dimesional (3D) imaging of phase microscopic objects. Classical and holographic methods of phase, interference and polarization contrast are discussed. Combination of the developed holographic methods with the methods of digital image processing allowed creating the digital holographic interference microscope (DHIM). The first 3D images of native phase microscopic objects such as blood cells were obtained using the DHIM. The results of DHIM application for study of blood erythrocytes, thin films, micro-crystals are presented.
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Chapter 1 Classical Microscopy and Methods of Phase Microscopic Objects Visualization
Chapter 2 Holography and Holographic Microscopy
Chapter 3 Holographic Methods of Phase Microscopic Object Imaging and the Digital Holographic Interference Microscope
Chapter 4 Application of the Digital Holographic Interference Microscope for Blood Cells Study
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3D morphology acryl substrate amplitude anisotropic anisotropic microscopic objects background blood smears classical microscopy coherent noise contrast method decrease DHIM diffracted digital holographic interference erythrocyte morphology erythrocyte shapes finite width fragment fringes of finite functionality hologram holographic interference microscope holographic interferometry holographic methods holographic microscope holographic phase-contrast method human blood erythrocytes hypoxia image plane images of phase intensity interference fringes interference pattern interferograms interferometry in fringes investigation laser light mean sphericity coefficient method of holographic method of interferometry microscopic objects visualization microscopy method modifications of blood morphological modifications numerical aperture object under study obtaining contrast images optical resolution oxygen capacity ozone therapy patients phase difference phase increment inserted phase microscopic objects phase-contrast images polarization-contrast possible radiation therapy recorded and reconstructed reference beam reference wave refractive indices refractive-index Safronov sickle-cell disease specimen spherocyte thickness thin films Tishko Titar Vestn visualization of phase wave transmitted wavelength